Composite

Part:BBa_K4591012:Design

Designed by: XinTong Shen   Group: iGEM23_ZJUT-China   (2023-10-08)


P43-DepoPETase-MHETase-T500


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 421
    Illegal XhoI site found at 505
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 421
    Illegal PstI site found at 588
    Illegal NgoMIV site found at 275
    Illegal NgoMIV site found at 2010
    Illegal AgeI site found at 1230
    Illegal AgeI site found at 1347
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pgrac_rbs_tetR_T500_PdegQ_3tetO_rbs_sfGFP_T500


Source

pgrac_rbs_tetR_T500_PdegQ_3tetO_rbs_sfGFP_T500

References